Journal: Molecular Cell

Article Title: Global analysis of protein-RNA interactions in SARS-CoV-2-infected cells reveals key regulators of infection

doi: 10.1016/j.molcel.2021.05.023

Figure Lengend Snippet:

Article Snippet: compound 3k , BIOZOL , SEL-S8616.

Techniques: Immunofluorescence, Western Blot, Recombinant, Software

Journal: bioRxiv

Article Title: Global analysis of protein-RNA interactions in SARS-CoV-2 infected cells reveals key regulators of infection

doi: 10.1101/2020.11.25.398008

Figure Lengend Snippet: A) Proteins with identified phenotypes in genome-wide screens using viruses. Proteins dowregulated in the cRIC experiment are displayed along the x axis, while y axis indicates the number of screens in which the protein has caused a phenotype in infection. B) Proportion of proteins within the cRIC and vRIC datasets that have been linked to infection using Pudmed automatized analysis. C) Comparison of RBPs upregulated by cRIC or/and present in the vRIC dataset to drug databases. D) Effect of PKM2 inhibitor on SARS-Cov-2 infection. Red line indicates the effects in infection measured by protein ELISA at each drug dose. Black line shows cell viability at each drug dose. Error bars are SEM from three independent experiments. E) Effects of DDX1 and FAM98A knock down in the tRNA-LC subunits and SARS-CoV-2 NCAP. Data is normalised to wild type cells. Error bars represent standard deviation using information from three biological replicates. *, p< 0.05; **, p<0.01 and *** p<0.001.

Article Snippet: Dilutions ranged from 2.5 nM to 50 µM for Ro 08-2750 (TOCRIS, #2272) and the BTYNB IMP1 inhibitor (Cayman Chemical, #25623), 5 nM to 100 µM for Ganetespib (BIOZOL, BYT-ORB181166) and MS-444 (Hycultec, HY100685-1mg) and 1,25 nM to 25 µM for the PKM2 inhibitor - compound 3k (BIOZOL, SEL-S8616)).

Techniques: Genome Wide, Infection, Enzyme-linked Immunosorbent Assay, Standard Deviation